Reproducible and Reliable
Optical labels reduce target-probe binding efficiency and specificity reducing result consistency between experiments. Additionally, variations in silicon or glass substrates randomly scatter light; hence further reducing repeatability between experiments.
Over their lifetime, light sources and detectors vary in intensity and sensitivity, respectively, requiring routine calibration. Furthermore, users perceive the intensity and color of each fluorescent spot differently. This subjective interpretation of the same result adds to variation between tests.
Variability between experiments inhibits comparison between two slides or between spots on the same slide.
The SET platform provides a more reliable and consistent platform upon which to explore molecular interactions. Because the SET is a solid-state device, built using conventional CMOS processes, each transistor functions identically to all others on the array. Conventional spotting methods verify probe activity prior immobilization on the array, ensuring results match the biological question of interest.
The SET detection method removes subjective interpretation and background noise. The SET's electronic signal is a direct indication the probe target binding reaction. Furthermore, there is no background noise from non-specific binding to the silicon substrate, affording meaningful comparison across and between arrays.
QLD's SET technology is self normalizing, accounting for any slight variation in fabrication between test sites. Additionally, QLD has proprietary real time data analysis methods accounting for minor variations in particle and probe size without discarding data.